Introducing the HCR™ HiFi Encoder

In immunofluorescence (IF), flexibility often comes at the expense of performance. Traditional secondary antibody workflows constrain researchers by restricting the use of same-species primary antibodies and relying upon relatively weak signal amplification. Direct IF with labeled primary antibodies simply does not offer robust enough performance, despite offering more freedom with primary selection.

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The HCR™ HiFi Encoder fundamentally changes conventional approaches.

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The HCR™ HiFi Encoder is an innovative plug-and-play reagent that lets you rapidly encode any unmodified primary antibody with the ability to trigger HCR™ Gold Amplifiers without purification, conjugation, or reformulation. By working with any off-the-shelf primary, the HCR™ HiFi Encoder frees you from fixed antibody panels and lets you design experiments around the primary antibodies you already know and trust.

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What does that mean for you?

• Keep using the primaries you already have validated.
• Use multiple same-species primaries in parallel.
• Achieve best-in-class HCR™ amplification, up to 50× brighter than direct-labeled secondaries.

It's flexible, it's fast, and it's built for real-world research. Explore the workflow and key FAQs in our Quick Reference Guide.