All major automation platforms can run HCR™ RNA-CISH

Using HCR™ RNA-CISH, researchers can detect any RNA target in any FFPE tissue sample with an unparalleled combination of performance, speed, and versatility on popular automation platforms. HCR™ RNA-CISH has been highly optimized to eliminate all enzyme/protease digestion, enabling (1) tissue morphology preservation, (2) native compatibility with all IHC assays, and (3) straightforward image analysis.

Key features of HCR™ RNA-CISH include: 

• Runs completed as fast as 6 hours
• Clean and consistent signal intensity
• Native support with IHC
• Conserved tissue morphology

The HCR™ RNA-CISH Workflow

HCR™ RNA-CISH features automatic background suppression and a high signal-to-background ratio for all FFPE tissue sections.

Clean and Consistent Signal Intensity

HCR™ RNA-CISH is significantly more sensitive than other RNA-ISH methods, without any sacrifice in specificity.

Native Support for IHC

No protease treatment is required with HCR™ RNA-CISH, enabling high-definition RNA and protein co-detection without compromising protein integrity or tissue morphology. Please review our blog post about duplex HCR RNA-CISH + IHC here for additional information.

Conserved Tissue Morphology

With HCR™ RNA-CISH, nuclear and tissue integrity are consistent and conserved throughout your sample because of the milder reaction conditions. With RNAscope, tissue morphology may be heavily compromised due to harsh enzyme pretreatment.

HCR™ RNA-CISH Images on the Roche DISCOVERY and Leica BOND Autostainers

Discovery Red Chromogen (Left) and Leica Fast Red Chromogen (Right): 

Discovery ChromoMap DAB Chromogen (Left) and Leica Brown DAB Chromogen (Right): 

Discovery Purple Chromogen: