The MI Team
Using HCR™ RNA-CISH, researchers can detect any RNA target in any FFPE tissue sample with an unparalleled combination of performance, speed, and versatility on popular automation platforms. HCR™ RNA-CISH has been highly optimized to eliminate all enzyme/protease digestion, enabling (1) tissue morphology preservation, (2) native compatibility with all IHC assays, and (3) straightforward image analysis.
Key features of HCR™ RNA-CISH include:
HCR™ RNA-CISH features automatic background suppression and a high signal-to-background ratio for all FFPE tissue sections.
HCR™ RNA-CISH is significantly more sensitive than other RNA-ISH methods, without any sacrifice in specificity.
No protease treatment is required with HCR™ RNA-CISH, enabling high-definition RNA and protein co-detection without compromising protein integrity or tissue morphology. Please review our blog post about duplex HCR RNA-CISH + IHC here for additional information.
With HCR™ RNA-CISH, nuclear and tissue integrity are consistent and conserved throughout your sample because of the milder reaction conditions. With RNAscope, tissue morphology may be heavily compromised due to harsh enzyme pretreatment.
Discovery Red Chromogen (Left) and Leica Fast Red Chromogen (Right):
Discovery ChromoMap DAB Chromogen (Left) and Leica Brown DAB Chromogen (Right):
Discovery Purple Chromogen: