Written by
The MI Team
Third-Party Chromogen Compatibility on the Leica BOND RX/RXm
Although we recommend users take advantage of first-party chromogens available from Leica Biosystems, there may be workflows that benefit from the use of third-party chromogens. Our Product Development team continuously evaluates and validates third-party chromogens for use with HCR™ RNA-ISH on the Leica BOND RX/RXm. Below you can find our team's latest recommendations, and our HCR™ RNA-ISH Setup Guide for the BOND RX/RXm for using third-party chromogens can be found here.
Validated third-party HRP-driven chromogens (last updated: 11/13/23):
- Mixing Ratio: 1:20
- Deposition Time: 5-8 minutes
Biocare's Vina Green Chromogen
- Mixing Ratio: 1:20
- Deposition Time: 5-10 minutes
- Mixing Ratio: 1:50
- Deposition Time: 5-10 minutes
Enzo's HIGHDEF Yellow IHC Chromogen
- Mixing Ratio: 1:50
- Deposition Time: 10-15 minutes
Validated third-party AP-driven chromogens (last updated: 11/13/23):
Enzo's HIGHDEF Red IHC Chromogen
- Mixing Ratio: 1:50
- Deposition Time: 10-15 minutes
Biocare's Vulcan Red Chromogen
- Mixing Ratio: 1:50
- Deposition Time: 10-14 minutes
- Mixing Ratio: 1:50
- Deposition Time: 5-7 minutes
